LIPASES & LIPIDS
Structure, function and biotechnological applications
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The international conference "Lipases and Lipids: Structure, Function and Biotechnological Applications" was held in Santorini, Greece from the 6th to 8th of May 1999. The main purpose of the conference was to bring together the most relevant information on lipases and lipids. The recent data and future trends were discussed by leading scientists in the field.
The study of lipases and lipids is remarkably interdisciplinary, a fact that underlies the interest of meetings such as the Lipases and Lipids Conferences. In the Santorini Conference, scientists of different backgrounds, from basic to industrial and biomedical research, converged on a common objective: advancing scientific knowledge as it relates to the multiple features of lipases and lipids. As shown in the contents, the contributions in this book are grouped under six general headings in order to help the reader locate easily the manuscript of his interest. These six topics are:
― Structure and function of lipases
― Physicochemical behavior of lipids and its influence on lipase activity
― Expression and regulation of lipases
― Lipases in organic synthesis - Lipase selectivity
― Lipases and lipids of biomedical interest
― Biotechnological applications of lipases

Τρέχουσα έκδοση
12/
2000
ISBN
978-960-524-111-7
Τιμή καταλόγου
48,00

Περιεχόμενα

STRUCTURE AND FUNCTION OF LIPASES
A. Roussel, C. Whithers-Martinez, S. Canaan, F. Carriere, R. Verger and C. Cambillau
, Structure / Function Studies of Mammalian Digestive Lipases

J. Pleiss, M. Fischer, H. Scheib, T. Schulz and R. D. Schmid, Modelling Structure-Function Relationship of Lipase Binding Sites-a Case Study of Specificity and Selectivity

S. Bezzine, H. Chahinian, R. Verger and F. Carriere, The Lid Domain of Human Pancreatic Lipase is not Essential for Interfacial Binding and Functional Lipase-colipase Interactions

F. Beisson, N. Miled, J. de Caro, A. de Caro, V. Arondel and R. Verger, Interfacial Catalysis by Lipases

S. Glad, S. Patkar, J. Vind, K. Borch, L. Krogsoe and A. Svendsen, Characterisation of Tryptophan Variants from Humicola lanuginose Lipase

P. Weigel, V. Tlapak-Simmons and E. Kempner, Lipid Requirements of Hyaluronan Synthase Enzymes Revealed by Radiation Inactivation Analysis

PHYSICOCHEMICAL BEHAVIOR OF LIPIDS AND ITS INFLUENCE ON LIPASE ACTIVITY
A. Moren and A. Khan
, Phase Behavior and Characterisation of Protein-Surfactant Aggregates by NMR and Cryo-TEM Techniques

K. Balashev, L. Nielsen, T. Callisen, A. Svendsen and T. Bjornholm, AFM Visualisation of Lipid Bilayer Degradation due to Enzyme Action of Phospholipase A2 and Humicula Lanuginosa Lipase

T. Jensen, K. Kjaer, P. Howes, A. Svendsen, K. Balashev, N. Reitzel and T. Bjornholm, Model Systems for Biological Membranes Investigated by Grazing-incidence X-ray Diffraction and Specular Reflectivity

EXPRESSION AND REGULATION OF LIPASES
D. D’Agostino and M. Lowe
, Intestinal Expression of Pancreatic Lipase Related Protein 2

F. Cardenas, E. Alvarez, S. Elson, J. Sanchez-Montero and J. Sinisterra, Influence of the Chemical Structure of the Inducer in the Production of Extracellular Lipase by a Pseudomonas Cepacia Strain

M. Droge, F. Pries and W. Quax, Directed Evolution of Bacillus Lipase

LIPASES IN ORGANIC SYNTHESIS-LIPASE SELECTIVITY
J. Jongejan and A. Overbeeke
, Rational Design of Enantioselective Biotransformations in Organic Media. Towards a Comprehensive Model for Solvent-induced Changes of Lipase Enantioselectivity

M. van Kampen, H. Verheij and M. Egmond, Modifying the Substrate Specificity of Staphylococcal Lipases

M. Terreni, M. Pregnolato, I. Fuentes, G. Pagani, P. Moro, J. Guisan, P. Sabuquillo, R. Fernandez-Lafuente, Resolution of (±)-Glycidol by Using Lipases Immobilised on Hydrophobic Solid Support

LIPASES AND LIPIDS OF BIOMEDICAL INTEREST
A. Chiou,, T. Markidis, V. Constantinou-Kokotou, R. Verger and G. Kokotos
, Synthesis of Lipophilic α-Keto Amides and Study of their Inhibition Effect on Pancreatic and Gastric Lipases

K. Pekari and R. Schmidt, Recent Results in Glycosyl Phosphatidyl Inositol (GPI) Anchor Synthesis

E. Kitsiouli, G. Nakos and M. Lekka, Fluorimetric Determination of Phospholipase A2 and PAF-acetylhydrolase in Biological Fluids: the Effect of Protein

G. Leondaritis, V. Kapetaniou and D. Galanopoulou, Study of Phosphatidylinositol Hydrolyzing Activities in a Unicellular Eukaryote

A. Miller, Progress Towards Third Generation Non-Viral Vectors for Gene Therapy

C. Noula, P. Bonzom, A. Nicolaou and W. Gibbons, Analysis of Human Blood Platelet Lipids by 1H-NMR Spectroscopy: Exploration of a Possible Link With Coronary Artery Disease

A. Chroni and M. Mavri-Vavayanni, Purification and Characterisation of PAF-Acetylhydrolase from Rat Adipocytes

BIOTECHNOLOGICAL APPLICATIONS OF LIPASES
S. Minning, H. Scheib, P. Traub, J. Schmitt and R. Schmid
, Towards a Toolbox of Recombinant Microbial Lipases

Y. Shimada, A. Sugihara and Y. Tominaga, Enzymatic Production of Structured Lipids Containing Polyunsaturated Fatty Acids

V. Sereti, H. Stamatis and F. Kolisis, Strategies for the Lipase-Catalyzed Regioselective Acylation of Polyols in Organic Media

N. Gandhi and K. Mukherjee, Structured Lipids – Tailor-Made Using Lipase from Papaya Latex

C. Carvalho, M. Aires-Barros and J. Cabral, Cutinase Stabilization in Reversed Micelles: an Overview

S. Papanikolaou, I. Chevalot, G. Aggelis, M. Komaitis and I. Marc, Production of a Cocoa-butter Substitute from Industrial Fats by Yarrowia Lipolytica

P. Ferrer, F. Valero and C. Sola, Microbial Production of Native and Recombinant Tailor-Made Enzymes for Fine Chemicals Production

J. Pleiss, M. Fischer, H. Scheib, T. Schulz and R. D. Schmid, Modelling Structure-Function Relationship of Lipase Binding Sites-a Case Study of Specificity and Selectivity

S. Bezzine, H. Chahinian, R. Verger and F. Carriere, The Lid Domain of Human Pancreatic Lipase is not Essential for Interfacial Binding and Functional Lipase-colipase Interactions

F. Beisson, N. Miled, J. de Caro, A. de Caro, V. Arondel and R. Verger, Interfacial Catalysis by Lipases

S. Glad, S. Patkar, J. Vind, K. Borch, L. Krogsoe and A. Svendsen, Characterisation of Tryptophan Variants from Humicola lanuginose Lipase

P. Weigel, V. Tlapak-Simmons and E. Kempner, Lipid Requirements of Hyaluronan Synthase Enzymes Revealed by Radiation Inactivation Analysis

PHYSICOCHEMICAL BEHAVIOR OF LIPIDS AND ITS INFLUENCE ON LIPASE ACTIVITY
A. Moren and A. Khan
, Phase Behavior and Characterisation of Protein-Surfactant Aggregates by NMR and Cryo-TEM Techniques

K. Balashev, L. Nielsen, T. Callisen, A. Svendsen and T. Bjornholm, AFM Visualisation of Lipid Bilayer Degradation due to Enzyme Action of Phospholipase A2 and Humicula Lanuginosa Lipase

T. Jensen, K. Kjaer, P. Howes, A. Svendsen, K. Balashev, N. Reitzel and T. Bjornholm, Model Systems for Biological Membranes Investigated by Grazing-incidence X-ray Diffraction and Specular Reflectivity

EXPRESSION AND REGULATION OF LIPASES
D. D’Agostino and M. Lowe
, Intestinal Expression of Pancreatic Lipase Related Protein 2

F. Cardenas, E. Alvarez, S. Elson, J. Sanchez-Montero and J. Sinisterra, Influence of the Chemical Structure of the Inducer in the Production of Extracellular Lipase by a Pseudomonas Cepacia Strain

M. Droge, F. Pries and W. Quax, Directed Evolution of Bacillus Lipase

LIPASES IN ORGANIC SYNTHESIS-LIPASE SELECTIVITY
J. Jongejan and A. Overbeeke
, Rational Design of Enantioselective Biotransformations in Organic Media. Towards a Comprehensive Model for Solvent-induced Changes of Lipase Enantioselectivity

M. van Kampen, H. Verheij and M. Egmond, Modifying the Substrate Specificity of Staphylococcal Lipases

M. Terreni, M. Pregnolato, I. Fuentes, G. Pagani, P. Moro, J. Guisan, P. Sabuquillo, R. Fernandez-Lafuente, Resolution of (±)-Glycidol by Using Lipases Immobilised on Hydrophobic Solid Support

LIPASES AND LIPIDS OF BIOMEDICAL INTEREST
A. Chiou,, T. Markidis, V. Constantinou-Kokotou, R. Verger and G. Kokotos
, Synthesis of Lipophilic α-Keto Amides and Study of their Inhibition Effect on Pancreatic and Gastric Lipases

K. Pekari and R. Schmidt, Recent Results in Glycosyl Phosphatidyl Inositol (GPI) Anchor Synthesis

E. Kitsiouli, G. Nakos and M. Lekka, Fluorimetric Determination of Phospholipase A2 and PAF-acetylhydrolase in Biological Fluids: the Effect of Protein

G. Leondaritis, V. Kapetaniou and D. Galanopoulou, Study of Phosphatidylinositol Hydrolyzing Activities in a Unicellular Eukaryote

A. Miller, Progress Towards Third Generation Non-Viral Vectors for Gene Therapy

C. Noula, P. Bonzom, A. Nicolaou and W. Gibbons, Analysis of Human Blood Platelet Lipids by 1H-NMR Spectroscopy: Exploration of a Possible Link With Coronary Artery Disease

A. Chroni and M. Mavri-Vavayanni, Purification and Characterisation of PAF-Acetylhydrolase from Rat Adipocytes

BIOTECHNOLOGICAL APPLICATIONS OF LIPASES
S. Minning, H. Scheib, P. Traub, J. Schmitt and R. Schmid
, Towards a Toolbox of Recombinant Microbial Lipases

Y. Shimada, A. Sugihara and Y. Tominaga, Enzymatic Production of Structured Lipids Containing Polyunsaturated Fatty Acids

V. Sereti, H. Stamatis and F. Kolisis, Strategies for the Lipase-Catalyzed Regioselective Acylation of Polyols in Organic Media

N. Gandhi and K. Mukherjee, Structured Lipids – Tailor-Made Using Lipase from Papaya Latex

C. Carvalho, M. Aires-Barros and J. Cabral, Cutinase Stabilization in Reversed Micelles: an Overview

S. Papanikolaou, I. Chevalot, G. Aggelis, M. Komaitis and I. Marc, Production of a Cocoa-butter Substitute from Industrial Fats by Yarrowia Lipolytica

P. Ferrer, F. Valero and C. Sola, Microbial Production of Native and Recombinant Tailor-Made Enzymes for Fine Chemicals Production

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